GRF2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88266

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRF2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRF2 Antibody - BSA Free

Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.
GRF2 Antibody - BSA Free Immunohistochemistry-Paraffin: GRF2 Antibody - BSA Free [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody - BSA Free [NBP1-88266]

Staining of human placenta shows moderate cytoplasmic positivity in endothelial cells.
GRF2 Antibody - BSA Free Immunohistochemistry-Paraffin: GRF2 Antibody - BSA Free [NBP1-88266]

Immunohistochemistry-Paraffin: GRF2 Antibody - BSA Free [NBP1-88266]

Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
GRF2 Antibody - BSA Free Western Blot: GRF2 Antibody - BSA Free [NBP1-88266]

Western Blot: GRF2 Antibody - BSA Free [NBP1-88266]

Analysis in control (vector only transfected HEK293T lysate) and RAPGEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for GRF2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRF2

GRF2 encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Alternate Names

C3GGRF2guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene), CRK SH3-binding GNRP, DKFZp781P1719, Guanine nucleotide-releasing factor 2, Protein C3G, Rap guanine nucleotide exchange factor (GEF) 1, rap guanine nucleotide exchange factor 1

Gene Symbol

RAPGEF1

Additional GRF2 Products

Product Documents for GRF2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GRF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GRF2 Antibody - BSA Free

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Protocols

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