GRIM19 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03756

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRIM19 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human GRIM19 (NP_057049.5). MAASKVKQDMPPPGGYGPIDYKRNLPRRGLSGYSMLAIGIGTLIYGHWSIMKWNRERRRLQIEDFEARIALLPLLQAETDRRTLQMLRENLEEEAIIMKDVPDWKVGESVFHTTRWVPPLIGELYGLRTTEEALHASHGFMWYT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRIM19 Antibody - BSA Free

GRIM19 Antibody - Azide and BSA Free

Western Blot: GRIM19 Antibody - Azide and BSA Free [GRIM19] -

Western Blot: GRIM19 Antibody - Azide and BSA Free [GRIM19] - Western blot analysis of lysates from wild type(WT) and GRIM19 knockdown (KD) 293T cells, using [KD Validated] GRIM19 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
GRIM19 Antibody - Azide and BSA Free

Western Blot: GRIM19 Antibody - Azide and BSA Free [GRIM19] -

Western Blot: GRIM19 Antibody - Azide and BSA Free [GRIM19] - Western blot analysis of various lysates, using [KD Validated] GRIM19 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
GRIM19 Antibody - Azide and BSA Free

Western Blot: GRIM19 Antibody - Azide and BSA Free [GRIM19] -

Western Blot: GRIM19 Antibody - Azide and BSA Free [GRIM19] - Western blot analysis of lysates from wild type (WT) and GRIM19 knockout (KO) HeLa cells, using [KO Validated] GRIM19 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
GRIM19 Antibody - Azide and BSA Free

Western Blot: GRIM19 Antibody - Azide and BSA Free [GRIM19] -

Western Blot: GRIM19 Antibody - Azide and BSA Free [GRIM19] - Western blot analysis of various lysates using GRIM19 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for GRIM19 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRIM19

The GRIM19 gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transf

Alternate Names

B16.6, CDA016, Cell death regulatory protein GRIM-19, cell death-regulatory protein GRIM19, CI-B16.6, complex I B16.6 subunit, Complex I-B16.6, FLJ58045, FLJ59191, Gene associated with retinoic and IFN-induced mortality 19 protein, Gene associated with retinoic and interferon-induced mortality 19 protein, GRIM-19CGI-39, GRIM19NADH-ubiquinone oxidoreductase B16.6 subunit, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Gene Symbol

NDUFA13

Additional GRIM19 Products

Product Documents for GRIM19 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GRIM19 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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