Growth Cone Antibody (2G13) - BSA Free

Novus Biologicals | Catalog # NB600-785

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Chicken

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 2G13

Format

BSA Free
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Product Specifications

Immunogen

Embryonic chick tectal membranes

Reactivity Notes

Predicted cross-reactivities: Rat, Mouse

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This is useful as a general reagent for the detection of axonal growth cones in tissue sections and potentially a key marker in neuronal development research.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Growth Cone Antibody (2G13) - BSA Free

Immunohistochemistry-Paraffin: Growth Cone Antibody (2G13) [NB600-785]

Immunohistochemistry-Paraffin: Growth Cone Antibody (2G13) [NB600-785]

Immunohistochemistry-Paraffin: Growth Cone Antibody (2G13) [NB600-785] - Staining of growth cones of primary olfactory neurons within the olfactory bulb in the developing rat brain using NB 600-785.

Applications for Growth Cone Antibody (2G13) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
This antibody can be used in Immunohistochemistry. *Optimal dilutionsshould be determined by the end user. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. This antibody does not work in Flow Cytometry.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue Culture Supernatant

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Growth Cone

This antibody recognises an intracellular protein 2G13p which is restricted to growth cones in developing brain and embryonic neurons in culture. The protein 2G13p also appears to be associated with filamentous actin in the growth cones suggesting a role for 2G13p in growth cone motility.This antibody is suitable for use in immunohistological studies of both frozen and paraffin-embedded sections, staining growth cones, including their filopodia and lamellipodies.

Additional Growth Cone Products

Product Documents for Growth Cone Antibody (2G13) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Growth Cone Antibody (2G13) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Growth Cone Antibody (2G13) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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