Fish Growth Hormone ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-21153

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

312.5 pg/mL

Assay Range

312.5 pg/mL - 5000 pg/mL
View all Growth Hormone ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Fish

Specificity

This assay has high sensitivity and excellent specificity for detection of fish Growth Hormone. No significant cross-reactivity or interference between fish Growth Hormone and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 15

Recovery for Fish Growth Hormone ELISA Kit (Colorimetric)

Recovery

90 - 98%

Scientific Data Images for Fish Growth Hormone ELISA Kit (Colorimetric)

Fish Growth Hormone ELISA Kit (Colorimetric)

Fish Growth Hormone ELISA Kit (Colorimetric) [NBP3-21153] -

Fish Growth Hormone ELISA Kit (Colorimetric) [NBP3-21153] - Standard Curve Reference

Kit Contents for Fish Growth Hormone ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • Conjugate
  • HRP-avidin
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Growth Hormone

Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression.

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Gene Symbol

GH1

Additional Growth Hormone Products

Product Documents for Fish Growth Hormone ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Fish Growth Hormone ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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