GRP75/HSPA9B/Mortalin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89729

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRP75/HSPA9B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGT

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25379943).

Marker

Immortalization Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRP75/HSPA9B/Mortalin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729]

Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729]

Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
GRP75/HSPA9B/Mortalin Antibody - BSA Free Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Staining of human colon shows strong positivity in mitochondria in glandular cells.
GRP75/HSPA9B/Mortalin Antibody - BSA Free Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Staining of human liver shows strong positivity in mitochondria in hepatocytes.
GRP75/HSPA9B/Mortalin Antibody - BSA Free Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Staining of human kidney shows strong positivity in mitochondria in cells in tubules.
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729]

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729]

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human cerebral cortex shows strong positivity in mitochondria in neurons.
GRP75/HSPA9B/Mortalin Antibody - BSA Free Western Blot: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Western Blot: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
GRP75/HSPA9B/Mortalin Antibody - BSA Free Western Blot: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Western Blot: GRP75/HSPA9B/Mortalin Antibody - BSA Free [NBP1-89729]

Analysis in human cell line CACO-2.

Applications for GRP75/HSPA9B/Mortalin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRP75/HSPA9B

The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery. They are involved in chaperoning nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Research indicates that members of the HSP 70 family may act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

CSA, HSPA9, HSPA9B, Mortalin-2, MOT2, PBP74

Gene Symbol

HSPA9

Additional GRP75/HSPA9B Products

Product Documents for GRP75/HSPA9B/Mortalin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GRP75/HSPA9B/Mortalin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

Citations for GRP75/HSPA9B/Mortalin Antibody - BSA Free

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Protocols

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FAQs

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