GTF2A1 Antibody (2H5) - Azide and BSA Free

Novus Biologicals | Catalog # H00002957-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2H5

Format

Azide and BSA Free
View all GTF2A1 Primary Antibodies »

Product Specifications

Immunogen

GTF2A1 (NP_056943, 1 a.a. ~ 63 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFHSEE

Localization

Nuclear

Specificity

GTF2A1 - general transcription factor IIA, 1, 19/37kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GTF2A1 Antibody (2H5) - Azide and BSA Free

Sandwich ELISA: GTF2A1 Antibody (2H5) [H00002957-M01] - Detection limit for recombinant GST tagged GTF2A1 is approximately 0.03ng/ml as a capture antibody.

Applications for GTF2A1 Antibody (2H5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GTF2A1

GTF2A1 bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human GTF2A1 consists of three subunits of 35 kDa (alpha subunit), 19 kDa (beta subunit) and 12 kDa (gamma subunit). The alpha and beta units are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (gamma subunit), retains native GTF2A1 activity.

Alternate Names

General transcription factor IIA subunit 1, general transcription factor IIA, 1, 19/37kDa, glucose regulated protein, 58kD pseudogene, MGC129969, MGC129970, TF2A1, TFIIA, TFIIA alpha, p55, TFIIA-42, TFIIAL, transcription initiation factor IIA subunit 1, Transcription initiation factor TFIIA 42 kDa subunit

Entrez Gene IDs

2957 (Human)

Gene Symbol

GTF2A1

OMIM

600520 (Human)

UniProt

NP_056943 (Human)

Additional GTF2A1 Products

Product Documents for GTF2A1 Antibody (2H5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GTF2A1 Antibody (2H5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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