GTF2IRD1 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-61055
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human general transcription factor 2I repeat domain containing 1 using the numbering given in entry NP_057412.1 (GeneID 9569).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GTF2IRD1 Antibody - BSA Free
Immunohistochemistry-Paraffin: GTF2IRD1 Antibody [NB100-61055]
Immunohistochemistry-Paraffin: GTF2IRD1 Antibody [NB100-61055] - Human prostate carcinoma. Antibody used at a dilution of 1:5,000.Immunohistochemistry-Paraffin: GTF2IRD1 Antibody [NB100-61055]
Immunohistochemistry-Paraffin: GTF2IRD1 Antibody [NB100-61055] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-GTF2IRD/TFII-IRD1 used at a dilution of 1:500.Immunoprecipitation: GTF2IRD1 Antibody [NB100-61055]
Immunoprecipitation: GTF2IRD1 Antibody [NB100-61055] - WB and Immunoprecipitation: GTF2IRD/TFII-IRD1 Antibody NB100-61055 - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-61055 used for IP at 3 ug/mg lysate. GTF2IRD1/TFII-IRD1 was also immunoprecipitated by rabbit anti-GTF2IRD1/TFII-IRD1 antibody NB100-60156, which recognizes a downstream epitope.Applications for GTF2IRD1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000- 1:5000
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GTF2IRD1
Alternate Names
BEN, Cream1, General transcription factor III, general transcription factor II-I repeat domain-containing protein 1, GTF2I repeat domain containing 1, GTF2I repeat domain-containing protein 1, GTF3GTF2I repeat domain-containing 1, hMusTRD1alpha1, Muscle TFII-I repeat domain-containing protein 1, muscle TFII-I repeat domain-containing protein 1 alpha 1, MusTRD1, MusTRD1/BEN, MUSTRD1general transcription factor 3, RBAP2WBSCR12binding factor for early enhancer, Slow-muscle-fiber enhancer-binding protein, USE B1-binding protein, WBS, WBSCR11, Williams-Beuren syndrome chromosomal region 11 protein, Williams-Beuren syndrome chromosomal region 12 protein, Williams-Beuren syndrome chromosome region 11
Entrez Gene IDs
9569 (Human)
Gene Symbol
GTF2IRD1
UniProt
Additional GTF2IRD1 Products
Product Documents for GTF2IRD1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GTF2IRD1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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