gtf3c3 Antibody (3D9) - Azide and BSA Free

Novus Biologicals | Catalog # H00009330-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3D9

Format

Azide and BSA Free
View all gtf3c3 Primary Antibodies »

Product Specifications

Immunogen

GTF3C3 (NP_036218, 112 a.a. ~ 214 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TPEQPTAGDVFVLEMVLNRETKKMMKEKRPRSKLPRALRGLMGEANIRFARGEREEAILMCMEIIRQAPLAYEPFSTLAMIYEDQGDMEKSLQFELIAAHLNP

Reactivity Notes

Human. Other species not tested.

Specificity

GTF3C3 (3D9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for gtf3c3 Antibody (3D9) - Azide and BSA Free

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02]

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02]

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02] - GTF3C3 monoclonal antibody (M02), clone 3D9 Analysis of GTF3C3 expression in Hela S3 NE.
Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02]

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02]

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02] - Analysis of GTF3C3 over-expressed 293 cell line, cotransfected with GTF3C3 Validated Chimera RNAi ( Cat # H00009330-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF3C3 monoclonal antibody (M02), clone 3D9 (Cat # H00009330-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02]

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02]

Western Blot: gtf3c3 Antibody (3D9) [H00009330-M02] - Analysis of GTF3C3 expression in transfected 293T cell line by GTF3C3 monoclonal antibody (M02), clone 3D9.Lane 1: GTF3C3 transfected lysate(101.3 KDa).Lane 2: Non-transfected lysate.

Applications for gtf3c3 Antibody (3D9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500RNAi Validation
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. It has also been used for ELISA and RNAi validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: gtf3c3

General transcription factor 3C polypeptide 3 (GTF3C3/TFIIIC102) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C3/TFIIIC102 appears to be important for stable complex formation and recruitment of RNA polymerase III by interacting with TFIIIB90, TBP, and TFIIIC63. GTF3C3/TFIIIC102 has been shown to also interact with DEDD and FLAME-3 proteins. This interaction may function to translocate GTF3C3/TFIIIC102 to the nucleus and regulate TFIIIC activity.

Alternate Names

general transcription factor 3C polypeptide 3, general transcription factor IIIC, polypeptide 3, 102kDa, TF3C-gamma, TFIIIC 102 kDa subunit, TFIIIC102general transcription factor IIIC, polypeptide 3 (102kD), TFiiiC2-102, TFIIICgamma, Transcription factor IIIC 102 kDa subunit, Transcription factor IIIC subunit gamma, transcription factor IIIC, 102 kD

Entrez Gene IDs

9330 (Human)

Gene Symbol

GTF3C3

OMIM

604888 (Human)

UniProt

NP_036218 (Human)

Additional gtf3c3 Products

Product Documents for gtf3c3 Antibody (3D9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for gtf3c3 Antibody (3D9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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