HA Tag Antibody (4810) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43714

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4810

Format

Azide and BSA Free
View all HA Tag Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to HA Tag

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for HA Tag Antibody (4810) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714]

Immunocytochemistry/ Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714]

Immunocytochemistry/Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714] - HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA stained by HA tag antibody [4810] diluted at 1:1000. Blue: Hoechst 33342 staining.
Western Blot: HA Tag Antibody (4810) [NBP2-43714]

Western Blot: HA Tag Antibody (4810) [NBP2-43714]

Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Human HEK293T cells overexpressed with Ly6K-HA. Dution 1:1000. Western blot image submitted by a verified customer review.
Flow Cytometry: HA Tag Antibody (4810) [NBP2-43714]

Flow Cytometry: HA Tag Antibody (4810) [NBP2-43714]

Flow Cytometry: HA Tag Antibody (4810) [NBP2-43714] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted from a verified customer review.
Western Blot: HA Tag Antibody (4810) [NBP2-43714]

Western Blot: HA Tag Antibody (4810) [NBP2-43714]

Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of 293T whole cell lysate/extract A: 20 ug whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C: Immunoprecipitation of HA tagged protein by 2.5 ug of HA tagg antibody 10% SDS-PAGE. The immunoprecipitated HA tagged protein was detected by HA tag antibody [4810] diluted at 1:3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.
Western Blot: HA Tag Antibody (4810) [NBP2-43714]

Western Blot: HA Tag Antibody (4810) [NBP2-43714]

Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of A: 30 ug HeLa whole cell lysate/extract B: 30 ug whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [4810] dilution: 1:5000.

Applications for HA Tag Antibody (4810) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Valdiation from verified customer review

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunoprecipitation

1:100 - 1:500

Western Blot

1:1000 - 1:10000

Reviewed Applications

Read 3 reviews rated 5 using NBP2-43714 in the following applications:

Flow Cytometry Panel Builder

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  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HA Tag

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Alternate Names

HA Epitope Tag

Additional HA Tag Products

Product Documents for HA Tag Antibody (4810) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HA Tag Antibody (4810) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

Citations for HA Tag Antibody (4810) - Azide and BSA Free

Customer Reviews for HA Tag Antibody (4810) - Azide and BSA Free (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • HA Tag Antibody (4810)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: HEK 293 cell line
    Species: Human
    Verified Customer | Posted 11/29/2021
    HEK 293 cell line
    HA Tag Antibody (4810) - Azide and BSA Free NBP2-43714
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: human HEK293T cells overexoressed with Ly6K-HA
    Species: Human
    Verified Customer | Posted 07/06/2016
    HA Epitope Tag Antibody (NBP2-43714) for flow cytometry
    HA Tag Antibody (4810) - Azide and BSA Free NBP2-43714
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: human HEK293T cells overexpressed with Ly6K-HA
    Species: Human
    Verified Customer | Posted 06/17/2016
    HA Epitope Tag Antibody (NBP2-43714)
    HA Tag Antibody (4810) - Azide and BSA Free NBP2-43714

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HA Tag Antibody (4810) - Azide and BSA Free

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  • Q: We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?

    A:

    We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

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