HA Tag Biotinylated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
BAM0601
Detection of HA-tagged Proteins by Western Blot.
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HA Tag Biotinylated Antibody Summary

Specificity
Detects N-terminal and C-terminal HA Tag proteins in Western blot.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1049F
Purification
Protein A or G purified from cell culture supernatant
Immunogen
HA Tag Peptide
Accession # ABB51961
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of HA-tagged Proteins by Western Blot. View Larger

Detection of HA-tagged Proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-HA Tag Biotinylated Monoclonal Antibody (Catalog # BAM0601) followed by Streptavidin-HRP (Catalog # DY998). Specific bands were detected for HA-tagged proteins at approximately 70 and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: HA Tag

Entrez Gene IDs
ABB51961.1 (Viral)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

Product Datasheets

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