HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71296

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4H2

Format

Azide and BSA Free
View all HAI-1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SPINT1(NP_857593) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free

Western Blot: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2)Azide and BSA Free [NBP2-71296]

Western Blot: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2)Azide and BSA Free [NBP2-71296]

Western Blot: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-71296] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGFA Inhibitor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HGFA
Immunocytochemistry/ Immunofluorescence: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-71296]

Immunocytochemistry/ Immunofluorescence: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-71296]

Immunocytochemistry/Immunofluorescence: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-71296] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HGFA Inhibitor 1.
Flow Cytometry: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-71296]

Flow Cytometry: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-71296]

Flow Cytometry: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-71296] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-HGFA Inhibitor 1 antibody, and then analyzed by flow cytometry.
Western Blot: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2)Azide and BSA Free [NBP2-71296]

Western Blot: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2)Azide and BSA Free [NBP2-71296]

Western Blot: HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-71296] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HGFA Inhibitor 1 monoclonal antibody.

Applications for HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:200

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HAI-1

SPINT1 - serine protease inhibitor, Kunitz type 1

Long Name

HGF Activator Inhibitor Type 1

Alternate Names

HAI1, SPINT1

Gene Symbol

SPINT1

Additional HAI-1 Products

Product Documents for HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HAI-1/HGFA Inhibitor 1 Antibody (OTI4H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Serine Proteases and Regulators

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Protocols

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FAQs

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