HBcAg Antibody (Hep2 (H69)) - BSA Free

Novus Biologicals | Catalog # NB100-64454

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Virus

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # Hep2 (H69)

Format

BSA Free
View all HBcAg Primary Antibodies »

Product Specifications

Immunogen

Purified hepatitis B core antigen

Specificity

NB100-64454 is specific for Hepatitis B core antigen (HBcAg).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Applications for HBcAg Antibody (Hep2 (H69)) - BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 30408957).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HBcAg

Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection. This antibody stains predominantly the nuclei of liver cells. Occasionally variable staining may also be seen in the perinuclear cytoplasm. Hepatitis B core antigen (HBcAg), the highly immunogenic 21kDa replicative form of the Hepatitis B virus (HBV), expressed by infected cells. Antibodies to HBcAg are produced by the host during and following an acute HBV infection and can be detected in chronic HBV carriers and patients recovered from HBV infection, usually persisting in the body for life.

Alternate Names

c, Core antigen, HBc, HBcAg, Hepatitis B Virus core antigen

Gene Symbol

C

Additional HBcAg Products

Product Documents for HBcAg Antibody (Hep2 (H69)) - BSA Free

Certificate of Analysis

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Product Specific Notices for HBcAg Antibody (Hep2 (H69)) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HBcAg Antibody (Hep2 (H69)) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HBcAg Antibody (Hep2 (H69)) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Is it possible to help me double confirm if these products contain any BSA? Catalog numbers: NB400-105, NB100-306, and NB100-64454.

    A: No, none of these antibodies have BSA in their formulation.

  • Q: We are interested in your HBc Ab (Cat# NB100-64454). Would you please provide the epitope of amino acid sequence of this Ab? The datasheet do not mention this information.

    A: This monoclonal antibody was raised against the purified hepatitis B core antigen (HBcAg, 1-183 a.a.). According to our database, HBcAg is the highly immunogenic 21 kDa replicative form of the Hepatitis B virus (HBV), expressed by infected Hep2 cells. Unfortunately, our lab has not mapped the epitope for this monoclonal antibody; therefore, there is not such information I can provide to you. Other available information about this clone is related to how it was produced and purified, for your reference: Spleen cells from immunized Balb/C mice were fused with cells of the SP2/0-Ag-14 myeloma cell line. Purified IgG was prepared by affinity chromatography and refined by S300 exclusion chromatography.

  • Q: Is it possible to help me double confirm if these products contain any BSA? Catalog numbers: NB400-105, NB100-306, and NB100-64454.

    A: No, none of these antibodies have BSA in their formulation.

  • Q: We are interested in your HBc Ab (Cat# NB100-64454). Would you please provide the epitope of amino acid sequence of this Ab? The datasheet do not mention this information.

    A: This monoclonal antibody was raised against the purified hepatitis B core antigen (HBcAg, 1-183 a.a.). According to our database, HBcAg is the highly immunogenic 21 kDa replicative form of the Hepatitis B virus (HBV), expressed by infected Hep2 cells. Unfortunately, our lab has not mapped the epitope for this monoclonal antibody; therefore, there is not such information I can provide to you. Other available information about this clone is related to how it was produced and purified, for your reference: Spleen cells from immunized Balb/C mice were fused with cells of the SP2/0-Ag-14 myeloma cell line. Purified IgG was prepared by affinity chromatography and refined by S300 exclusion chromatography.

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