HBsAg Antibody (HBsAg/7556R)

Novus Biologicals | Catalog # NBP3-23794

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # HBsAg/7556R
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Product Specifications

Immunogen

Recombinant full-length human HBsAg protein

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for HBsAg Antibody (HBsAg/7556R)

HBsAg Antibody (HBsAg/7556R)

Immunohistochemistry-Paraffin: HBsAg Antibody (HBsAg/7556R) [NBP3-23794] -

Formalin-fixed, paraffin-embedded human HBV-infected liver stained with HBsAg antibody (HBsAg/7556R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for HBsAg Antibody (HBsAg/7556R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HBsAg

Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

Alternate Names

HBsAg, HBV major surface antigen, HBV surface antigen, Hepatitis B Virus major surface antigen, Hepatitis B Virus Surface Antigen, Major surface antigen, protein S

Gene Symbol

S

Additional HBsAg Products

Product Documents for HBsAg Antibody (HBsAg/7556R)

Certificate of Analysis

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Product Specific Notices for HBsAg Antibody (HBsAg/7556R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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