Hck Antibody (2A6) - Azide and BSA Free

Novus Biologicals | Catalog # H00003055-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2A6

Format

Azide and BSA Free
View all Hck Primary Antibodies »

Product Specifications

Immunogen

HCK (AAH14435, 1 a.a. ~ 80 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQK

Localization

Isoform p60-HCK and isoform p59-HCK are associated with membranes. Isoform p60-HCK is also cytoplasmic.

Specificity

HCK - hemopoietic cell kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Hck Antibody (2A6) - Azide and BSA Free

Proximity Ligation Assay: Hck Antibody (2A6) [H00003055-M02]

Proximity Ligation Assay: Hck Antibody (2A6) [H00003055-M02]

Proximity Ligation Assay: Hck Antibody (2A6) [H00003055-M02] - Analysis of protein-protein interactions between FLT1 and HCK. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-HCK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: Hck Antibody (2A6) [H00003055-M02] - Detection limit for recombinant GST tagged HCK is approximately 3ng/ml as a capture antibody.

Applications for Hck Antibody (2A6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hck

The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

Long Name

Hemopoietic Cell Kinase

Alternate Names

Bmk, Hctk, JTK9

Entrez Gene IDs

3055 (Human)

Gene Symbol

HCK

OMIM

142370 (Human)

UniProt

AAH14435 (Human)

Additional Hck Products

Product Documents for Hck Antibody (2A6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Hck Antibody (2A6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hck Antibody (2A6) - Azide and BSA Free

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