HCN2 Antibody (S71-37) - BSA Free
Novus Biologicals | Catalog # NBP2-12895
![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895]](https://resources.rndsystems.com/images/products/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0010.jpg "Western Blot: HCN2 Antibody (S71-37) [NBP2-12895]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S71-37
Format
BSA Free
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Product Specifications
Immunogen
Fusion protein amino acids 761-863 of rat HCN2
Localization
Membrane
Specificity
Detects approx 95kDa. No cross-reactivity against HCN1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HCN2 Antibody (S71-37) - BSA Free
Western Blot: HCN2 Antibody (S71-37) [NBP2-12895]
Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Mouse Brain showing detection of ~95 kDa HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~95 kDa.![Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895]](https://resources.rndsystems.com/images/products/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0013.jpg "Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895]")
Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895]
Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN2 Antibody (D) Composite.![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895]](https://resources.rndsystems.com/images/products/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0012.jpg "Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895]")
Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895]
Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895]](https://resources.rndsystems.com/images/products/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0009.jpg "Western Blot: HCN2 Antibody (S71-37) [NBP2-12895]")
Western Blot: HCN2 Antibody (S71-37) [NBP2-12895]
Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.![Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895]](https://resources.rndsystems.com/images/products/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0006.jpg "Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895]")
Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895]
Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Human hippocampal tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.![Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895]](https://resources.rndsystems.com/images/products/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0014.jpg "Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895]")
Immunocytochemistry/ Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895]
Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 (NBP2-12895). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) HCN2 Antibody. (D) Hoechst (E) Composite.![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895]](https://resources.rndsystems.com/images/products/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0011.jpg "Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895]")
Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895]
Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.Applications for HCN2 Antibody (S71-37) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:1000
Application Notes
1 ug/ml of HCN2 Antibody was sufficient for detection of HCN2 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HCN2
Long Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Alternate Names
BCNG-2, BCNG2, EIG17, GEFSP11, HAC-1
Gene Symbol
HCN2
Additional HCN2 Products
Product Documents for HCN2 Antibody (S71-37) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HCN2 Antibody (S71-37) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for HCN2 Antibody (S71-37) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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