HCoV-229E Spike RBD Alexa Fluor™ Plus 488‑conjugated Antibody

Name: HCoV-229E Spike RBD Alexa Fluor™ Plus 488‑conjugated Antibody
Brand: R&D Systems
SKU: FAB11039AFP488
Price: 479 USD
Availability: InStock

R&D Systems | Catalog # FAB11039AFP488

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Product Specifications
Applications
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

HCoV-229E

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2840H

View all Spike RBD Primary Antibodies »

Product Specifications

Specificity

Detects both HCoV-229E and HCoV-NL63 in ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for HCoV-229E Spike RBD Alexa Fluor™ Plus 488‑conjugated Antibody

Application

Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: Spike RBD

HCoV-229E belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N). HCoV-229E is a member of the alpha-coronavirus family and was discovered in 1966 (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-NL63, HCoV-HKU1 and HCov-OC43, plus three viruses that caused the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2). HCov-229E Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. As with most coronaviruses, proteolytic cleavage of the S protein generates two distinct peptides, S1 and S2 subunits. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion. Although HCoV-229E S protein shares high homology (56%) with HCoV-NL63, it does not employ Angiotensin-Converting Enzyme 2 (ACE2) as the receptor like HCoV-NL63. Instead, HCoV-229E engages CD13 (aminopeptidase N) for cellular entry and replication (3). The receptor binding domain (RBD) of HCoV-229E is solely responsible for receptor binding through three extended receptor binding loops (4).

References

Hamre, D. and J.J. Procknow (1966) Proc. Soc. Exp. Biol. Med. 121:190.
Van der Hoek, L. et al. (2004) Nat. Med. 10:368.
Yeager, C.L. et al. (1992) Nature 357:420.
Wong, A.H.M. et al. (2017) Nat. Commun. 8:1735.

Long Name

Spike Receptor Binding Domain

Entrez Gene IDs

3200426 (HCoV-HKU1); 14254594 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

UniProt

P15423.1

Additional Spike RBD Products

Product Documents for HCoV-229E Spike RBD Alexa Fluor™ Plus 488‑conjugated Antibody

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Product Specific Notices for HCoV-229E Spike RBD Alexa Fluor™ Plus 488‑conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Infectious Diseases

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