HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-14062

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # PCRP-HDAC1-1B7

Format

Azide and BSA Free
View all Histone Deacetylase 1/HDAC1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human HDAC1 protein (Uniprot: Q13547)

Localization

Nucleus, Nucleoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13936).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free

Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free [NBP3-14062]

Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free [NBP3-14062]

Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free [NBP3-14062] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC1 Antibody (PCRP-HDAC1-1B7).
HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free [NBP3-14062] -

Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free [NBP3-14062] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free Flow Cytometry: HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free [NBP3-14062] -

Flow Cytometry: HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free [NBP3-14062] -

Flow cytometric analysis of PFA-fixed HeLa cells. HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications for HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 1/HDAC1

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Long Name

Histone Deacetylase 1

Alternate Names

GON-10, HD1, KDAC1, RPD3L1

Gene Symbol

HDAC1

Additional Histone Deacetylase 1/HDAC1 Products

Product Documents for HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HDAC1 Antibody (PCRP-HDAC1-1B7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Notch Pathway

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Protocols

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FAQs

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