HDAC11 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56382

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HDAC11 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed by immunizing mice with a synthetic peptide (AHQGNGHERDFMDDKRVYI) of human HDAC11.

Specificity

This peptide sequence is 100% homologous in multiple HDAC11 isoforms corresponding to amino acids 60-78. The observed molecular weight in western blots can vary depending on the isoform(s) detected. Users are encouraged to refer to NBCI data bases for more information about HDAC-11 isoforms and their amino acid size.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC11 Antibody - BSA Free

Western Blot: HDAC11 Antibody [NB100-56382]

Western Blot: HDAC11 Antibody [NB100-56382]

Western Blot: HDAC11 Antibody [NB100-56382] - Analysis of HDAC11 using this antibody. Brain lysate from A) human, B) mouse and C) rat brain probed with this antibody at 2 ug/ml.
Simple Western: HDAC11 Antibody [NB100-56382]

Simple Western: HDAC11 Antibody [NB100-56382]

Simple Western: HDAC11 Antibody [NB100-56382] - 1:25 dilution against the indicated concentration of protein lysate from human SVG-A cells. Image from verified customer review.

Applications for HDAC11 Antibody - BSA Free

Application
Recommended Usage

Simple Western

1:25

Western Blot

1-3 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Human SVG-A cell protein lysate, separated by Size, antibody dilution of 1:25

Reviewed Applications

Read 1 review rated 3 using NB100-56382 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HDAC11

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns. Recently, a new member of this family, HDAC11, has been cloned by Gao et al. HDAC11 transcripts were limited to kidney, heart, brain, skeletal muscle, and testis. These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Long Name

Histone deacetylase 11

Alternate Names

EC 3.5.1.98, HD11

Entrez Gene IDs

79885 (Human); 232232 (Mouse); 297453 (Rat)

Gene Symbol

HDAC11

UniProt

Q9UKV0 (Human)

Additional HDAC11 Products

Product Documents for HDAC11 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HDAC11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for HDAC11 Antibody - BSA Free (1)

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  • HDAC11 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: SVG-A whole cell lysate
    Species: Human
    Verified Customer | Posted 03/08/2019
    Antibody tested by Simple Western at 1:25 dilution against the indicated concentration of protein lysate from human SVG-A cells.
    HDAC11 Antibody - BSA Free NB100-56382

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