HDAC11 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16789

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

IF/IHC, Microarray

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HDAC11 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDAC11. The exact sequence is proprietary.

Reactivity Notes

Rat (89%), Bovine (88%)

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HDAC11 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HDAC11 Antibody [NBP2-16789]

Immunocytochemistry/ Immunofluorescence: HDAC11 Antibody [NBP2-16789]

HDAC11-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16789-img0009.jpg
Immunohistochemistry: HDAC11 Antibody [NBP2-16789]

Immunohistochemistry: HDAC11 Antibody [NBP2-16789]

HDAC11-Antibody-Immunohistochemistry-NBP2-16789-img0010.jpg
Simple Western: HDAC11 Antibody [NBP2-16789]

Simple Western: HDAC11 Antibody [NBP2-16789]

Simple Western: HDAC11 Antibody [NBP2-16789] - 1:25 dilution against the indicated concentration of protein lysate of human SVG-A cells. Image submitted by a verified customer review.
Knockdown Validated: HDAC11 Antibody [NBP2-16789]

Western Blot: HDAC11 Antibody [NBP2-16789]

HDAC11-Antibody-Knockdown-Validated-NBP2-16789-img0011.jpg
HDAC11 Antibody

Western Blot: HDAC11 Antibody [NBP2-16789] -

Western Blot: HDAC11 Antibody [NBP2-16789] - HDAC11 silencing decreases expression of downstream targets & abrogates self-renewal of CSCs. (A) Depletion of HDAC11 using two IPTG inducible shRNA shows a significant decrease in Sox2, YAP1 & Gli1 protein expression as compared to IPTG treated control shRNA. The glycolysis pathway targets HK2 & PDK2 also show reduced protein expression in the absence of HDAC11. No change is observed in PDK1 expression; the full images of the western blots are provided as Supplementary Fig. 4. (B) Quantitation of the western blot band intensities using ImageJ analysis. (C) Sphere formation assay with SP cells from shHDAC11 H1650 cells treated with IPTG show abrogation of self-renewal ability in the absence of HDAC11. (D) Quantitation of sphere formation assay reveal that IPTG treated SP cells from two different clones of HDAC11 shRNA containing H1650 form fewer spheres as compared to IPTG untreated or control shRNA containing cells. (E,F) Real time PCR analysis of H1650 cells with two different HDAC11 shRNA show a decrease in mRNA expression of Sox2, HK2, PDK1, PDK2 with IPTG treatment. No significant change was observed in Oct4 & Nanog expression. The depletion of HDAC11 was confirmed using RT-PCR. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32170113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for HDAC11 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Simple Western

1:25

Western Blot

1:500-1:3000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Human SVG-A cell protein lysate, separated by Size, antibody dilution of 1:25

Reviewed Applications

Read 1 review rated 4 using NBP2-16789 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HDAC11

This gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants.

Long Name

Histone deacetylase 11

Alternate Names

EC 3.5.1.98, HD11

Gene Symbol

HDAC11

UniProt

Q96DB2 (Human)

Additional HDAC11 Products

Product Documents for HDAC11 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HDAC11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for HDAC11 Antibody - BSA Free

Customer Reviews for HDAC11 Antibody - BSA Free (1)

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  • HDAC11 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: SVG-A whole cell lysate
    Species: Human
    Verified Customer | Posted 03/08/2019
    Antibody tested by Simple Western at 1:25 dilution against the indicated concentration of protein lysate of human SVG-A cells.
    HDAC11 Antibody - BSA Free NBP2-16789

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Protocols

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