HDAC6 Antibody (2H12) - BSA Free

Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] -

Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug).
Lane 3: HepG2 whole cell lysate (20ug).

Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] -

Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] - Image of HDAC6 Antibody (2H12) diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] -

Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate.
All lanes: HDAC6 Antibody at 1.1ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 132, 115 KDa
Observed band size: 160 KDa