Q: I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?

A: At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here

HE4/WFDC2 Antibody

Novus Biologicals | Catalog # NBP2-27299

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all HE4/WFDC2 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 1-50 of (human) HE4 was used as the immunogen for the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HE4/WFDC2 Antibody

Western Blot: HE4/WFDC2 Antibody [NBP2-27299]

Western Blot: HE4/WFDC2 Antibody [NBP2-27299] - Ovarian tumor line SKOV3 probed with HE4 antibody at 1 ug/ml.

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP2-27299]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP2-27299] - Formalin-fixed, paraffin-embedded normal breast tissue stained with HE4 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note ductal staining.

Applications for HE4/WFDC2 Antibody

Application

Recommended Usage

Immunohistochemistry-Paraffin

5-7ug/ml~

Western Blot

1-3ug/ml

Application Notes

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HE4/WFDC2

HE4, or human epididymis protein 4, is overexpressed in ovarian carcinomas (1-3). Ovarian cancer represents one disease where cure rate can be increased if the disease is diagnosed early. While the most routinely used serum marker is CA-125, this marker is elevated in only about 50% in patients in early disease stages. Conversely, by ELISA, HE4 detectable levels in serum appear far more frequently in patients with nonmalignant or early disease stages unlike CA-125. So whereas HE4 levels are elevated in about 90% of woman with ovarian cancer, HE4 is not frequently overexpressed as is CA-125 in patients with benign gynecologic diseases (4). Additionally, in conjunction with CA-125 and another serum marker, mesothelin, HE4 adds to a promising combination for earlier detection and diagnosis of ovarian cancer. Antibody to HE4 provides a useful tool for detection and potential quantization and further assessment of more in depth potential of HE4 as a biomarker in ovarian cancer.

Long Name

WAP Four-disulfide Core Domain protein 2

Alternate Names

EDDM4, WAP5, WFDC2

Gene Symbol

WFDC2

UniProt

Q14508 (Human)

Additional HE4/WFDC2 Products

Product Documents for HE4/WFDC2 Antibody

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Certificate of Analysis

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Product Specific Notices for HE4/WFDC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Serine Proteases and Regulators

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