Hemoglobin A2 Antibody (rHBA/9167)

Novus Biologicals | Catalog # NBP3-23944

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2b Kappa Clone # rHBA/9167
View all Hemoglobin A2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa1-100) of human Hemoglobin A2 protein (exact sequence is proprietary)

Localization

Extracellular space.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for Hemoglobin A2 Antibody (rHBA/9167)

Hemoglobin A2 Antibody (rHBA/9167) Immunohistochemistry-Paraffin: Hemoglobin A2 Antibody (rHBA/9167) [NBP3-23944] -

Immunohistochemistry-Paraffin: Hemoglobin A2 Antibody (rHBA/9167) [NBP3-23944] -

Immunohistochemistry-Paraffin: Hemoglobin A2 Antibody (rHBA/9167) [NBP3-23944] - Formalin-fixed, paraffin-embedded human kidney stained with Hemoglobin A2 Antibody (rHBA/9167). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for Hemoglobin A2 Antibody (rHBA/9167)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Hemoglobin A2

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta -pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) codingsequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but theydiffer significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA,which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains toconstitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemiasresult from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alphathalassemias have also been reported. (provided by RefSeq)

Alternate Names

alpha globin, alpha-2 globin, alpha-globin, Hemoglobin alpha chain, hemoglobin subunit alpha, hemoglobin, alpha 2

Gene Symbol

HBA2

Additional Hemoglobin A2 Products

Product Documents for Hemoglobin A2 Antibody (rHBA/9167)

Certificate of Analysis

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Product Specific Notices for Hemoglobin A2 Antibody (rHBA/9167)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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