Hepatocyte Specific Antigen Antibody (OCH1E5)
Novus Biologicals | Catalog # NBP2-34336
Key Product Details
Species Reactivity
Human, Canine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # OCH1E5
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Product Specifications
Immunogen
Extract of a formalin-fixed, rejected-allograft of a human liver
Localization
Finely granular cytoplasmic
Marker
Hepatocellular Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34699)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Hepatocyte Specific Antigen Antibody (OCH1E5)
![Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336]](https://resources.rndsystems.com/images/products/Hepatocyte-Specific-Antigen-Antibody-OCH1E5-Immunohistochemistry-Paraffin-NBP2-34336-img0004.jpg "Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336]")
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336]
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336] - Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Hepatocyte Specific Antigen Antibody (OCH1E5).![Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336]](https://resources.rndsystems.com/images/products/Hepatocyte-Specific-Antigen-Antibody-OCH1E5-Immunohistochemistry-Paraffin-NBP2-34336-img0003.jpg "Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336]")
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336]
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336] - Formalin-fixed, paraffin-embedded human Liver stained with Hepatocyte Specific Antigen Antibody (OCH1E5).
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336] -
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (OCH1E5) [NBP2-34336] - Hepatocellular carcinoma stained with HSA monoclonal antibodyApplications for Hepatocyte Specific Antigen Antibody (OCH1E5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Hepatocyte Specific Antigen
Alternate Names
Hepatocyte, HSA
Additional Hepatocyte Specific Antigen Products
Product Documents for Hepatocyte Specific Antigen Antibody (OCH1E5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Hepatocyte Specific Antigen Antibody (OCH1E5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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