Hexokinase 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-21294

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Hexokinase 2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ADQHRARQKTLEHLQLSHDQLLEVKRRMKVEMERGLSKETHASAPVKMLPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hexokinase 2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP3-21294] -

Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP3-21294] -

Staining of human cell line U-2 OS shows localization to cytosol & mitochondria.
Western Blot: Hexokinase 2 Antibody [NBP3-21294] -

Western Blot: Hexokinase 2 Antibody [NBP3-21294] -

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil

Applications for Hexokinase 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexokinase 2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Alternate Names

HK2, HKII, HXK2

Gene Symbol

HK2

Additional Hexokinase 2 Products

Product Documents for Hexokinase 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Hexokinase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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