HEY1 Antibody (HL2367) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25502

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2367 expressed in HEK293

Format

Azide and BSA Free
View all HEY1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human HEY1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HEY1 Antibody (HL2367) - Azide and BSA Free

HEY1 Antibody (HL2367) - Azide and BSA Free

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] -

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
HEY1 Antibody (HL2367) - Azide and BSA Free

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] -

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HEY1 Antibody (HL2367) - Azide and BSA Free

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] -

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HEY1 Antibody (HL2367) - Azide and BSA Free

Immunohistochemistry-Paraffin: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] -

Immunohistochemistry-Paraffin: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - HEY1 antibody [HL2367] detects HEY1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. HEY1 stained by HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HEY1 Antibody (HL2367) - Azide and BSA Free

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] -

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HEY1 Antibody (HL2367) - Azide and BSA Free

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] -

Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for HEY1 Antibody (HL2367) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HEY1

Hairy/enhancer of split-related proteins, such as HEY1, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. Hey1 functions as a corepressor, providing a mechanism of cross-talk between Notch and androgen signaling pathways. It is excluded from the nucleus in most human prostate cancers.

Alternate Names

basic helix-loop-helix protein OAF1, BHLHB31, Cardiovascular helix-loop-helix factor 2, CHF-2, CHF2hHRT1, Class B basic helix-loop-helix protein 31, Hairy and enhancer of split-related protein 1, hairy/enhancer-of-split related with YRPW motif 1, hairy/enhancer-of-split related with YRPW motif protein 1, Hairy-related transcription factor 1, HERP2HES-related repressor protein 1, HESR-1, HESR1MGC1274, HES-related repressor protein 2, HRT1, HRT-1OAF1

Gene Symbol

HEY1

Additional HEY1 Products

Product Documents for HEY1 Antibody (HL2367) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HEY1 Antibody (HL2367) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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