HGK/MAP4K4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84663

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HGK/MAP4K4 Antibody - BSA Free

Immunohistochemistry-Paraffin: HGK/MAP4K4 Antibody [NBP1-84663]

Immunohistochemistry-Paraffin: HGK/MAP4K4 Antibody [NBP1-84663]

Immunohistochemistry-Paraffin: HGK/MAP4K4 Antibody [NBP1-84663] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
HGK/MAP4K4 Antibody

Immunohistochemistry: HGK/MAP4K4 Antibody [NBP1-84663] -

Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, HES6, HMMR, HOXC6, HYAL1, IGF1, IQCK, MAP4K4, MKI67, PAGE4, PLIN2, PTEN, SIAH2, SMAD4, SOX9, SPP1, SYP, & TP53 from prostate cancer tissue microarrays. Scale bar represents 50 μm. Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-244), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
HGK/MAP4K4 Antibody - BSA Free Western Blot: HGK/MAP4K4 Antibody - BSA Free [NBP1-84663]

Western Blot: HGK/MAP4K4 Antibody - BSA Free [NBP1-84663]

Analysis in control (vector only transfected HEK293T lysate) and MAP4K4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407892).

Applications for HGK/MAP4K4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HGK

MAP4K4 is encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.

Long Name

Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase

Alternate Names

MAP4K4, MEKKK4

Gene Symbol

MAP4K4

Additional HGK Products

Product Documents for HGK/MAP4K4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HGK/MAP4K4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HGK/MAP4K4 Antibody - BSA Free

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Protocols

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