hHR23b Antibody (OTI7G10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71269

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7G10

Format

Azide and BSA Free
View all hHR23b Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-253 of human RAD23B (NP_002865) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for hHR23b Antibody (OTI7G10) - Azide and BSA Free

Western Blot: hHR23b Antibody (OTI7G10)Azide and BSA Free [NBP2-71269]

Western Blot: hHR23b Antibody (OTI7G10)Azide and BSA Free [NBP2-71269]

Western Blot: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY hHR23b.
Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269] - Analysis of Carcinoma of Human kidney tissue.. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269]

Immunohistochemistry: hHR23b Antibody (OTI7G10) - Azide and BSA Free [NBP2-71269] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for hHR23b Antibody (OTI7G10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: hHR23b

hHR23b is encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Alternate Names

hHR23B, HR23BUV excision repair protein RAD23 homolog B, p58, RAD23 (S. cerevisiae) homolog B, RAD23 homolog B (S. cerevisiae), RAD23, yeast homolog of, B, XP-C repair complementing complex 58 kDa, XP-C repair complementing protein, XP-C repair-complementing complex 58 kDa protein

Gene Symbol

RAD23B

Additional hHR23b Products

Product Documents for hHR23b Antibody (OTI7G10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for hHR23b Antibody (OTI7G10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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