HIC5/TGFB1I1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16828

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIC5/TGFB1I1. The exact sequence is proprietary.

Localization

Cell junction; focal adhesion; Nucleus matrix; Cytoplasm; cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIC5/TGFB1I1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HIC5/TGFB1I1 Antibody [NBP2-16828]

Immunocytochemistry/ Immunofluorescence: HIC5/TGFB1I1 Antibody [NBP2-16828]

Immunocytochemistry/Immunofluorescence: HIC5 Antibody [NBP2-16828] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using HIC5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
HIC5/TGFB1I1 Antibody - BSA Free

Western Blot: HIC5/TGFB1I1 Antibody [NBP2-16828] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HIC5 antibody [N1], N-term (NBP2-16828) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.
HIC5/TGFB1I1 Antibody - BSA Free

Western Blot: HIC5/TGFB1I1 Antibody [NBP2-16828] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HIC5 antibody [N1], N-term (NBP2-16828) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for HIC5/TGFB1I1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HIC5/TGFB1I1

This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hydrogen Peroxide-inducible Clone 5

Alternate Names

ARA55, TGFB1I1, TSC-5

Gene Symbol

TGFB1I1

UniProt

Additional HIC5/TGFB1I1 Products

Product Documents for HIC5/TGFB1I1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HIC5/TGFB1I1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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