Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, ELISA, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # His17

Format

BSA Free
View all His Tag Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with His6-Tag conjugated to BSA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for His Tag Antibody (His17) - BSA Free

Western Blot: His Tag Antibody (His17) [NB100-72978]

Western Blot: His Tag Antibody (His17) [NB100-72978]

Western Blot: His Tag Antibody (His17) [NB100-72978] - Human recombinant protein with HRP conjugated His-tag. Western blot image submitted by a verified customer review.
Simple Western: His Tag Antibody (His17) [NB100-72978]

Simple Western: His Tag Antibody (His17) [NB100-72978]

Simple Western: His Tag Antibody (His17) [NB100-72978] - Human purified protein. Simple Western image submitted by a verified customer review.

Applications for His Tag Antibody (His17) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Human purified protein, separated by Size

Reviewed Applications

Read 1 review rated 5 using NB100-72978 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Antibody (His17) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for His Tag Antibody (His17) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for His Tag Antibody (His17) - BSA Free (1)

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  • His Tag Antibody (His17)
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 12/04/2018
    His Tag Antibody (His17) - BSA Free NB100-72978

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FAQs for His Tag Antibody (His17) - BSA Free

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  • Q: Is this really against the HIS-tag of his-tagged proteins? The specificity and immunogen mentions a mouse monoclonal - not HIS-tag. Please, confirm the immunogen for this antibody.

    A: NB100-72978 is raised against His tag and is suitable to detect any His tagged protein.

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