Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, ELISA, Immunoprecipitation

Label

HRP

Antibody Source

Monoclonal Mouse IgG1 Clone # His17
View all His Tag Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with His6-Tag conjugated to BSA.

Reactivity Notes

6x His Tag specific

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for His Tag Antibody (His17) [HRP]

Western Blot: His Tag Antibody (His17) [HRP] [NB100-72978H] - Human recombinant protein with HRP conjugated His-tag. Western blot image submitted by a verified customer review.

Applications for His Tag Antibody (His17) [HRP]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB100-72978H in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Antibody (His17) [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for His Tag Antibody (His17) [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for His Tag Antibody (His17) [HRP] (2)

5 out of 5
2 Customer Ratings
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  • His Tag Antibody (His17) [HRP]
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human IgG
    Species: Mouse
    Verified Customer | Posted 10/27/2020
    Mapping Carbohydrate Protein interactions
    His Tag Antibody (His17) [HRP] NB100-72978H
  • His Tag Antibody (His17) [HRP]
    Name: Anonymous
    Application: Western Blot
    Sample Tested: recombinant protein with his-tag
    Species: recombinant protein with his-tag and Human
    Verified Customer | Posted 04/05/2019
    I use this antibody to check my purified recombinant protein by western blot. Because it is conjugated with HRP, no secondary antibody is needed, so to save hours of time. Low/none background. Very happy with the purchase.
    His Tag Antibody (His17) [HRP] NB100-72978H

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