His Tag Antibody (RMH01) - Chimeric

Name: His Tag Antibody (RMH01) - Chimeric
Brand: Novus Biologicals
SKU: NBP2-61478
Price: 479 USD
Availability: InStock
Rating: 5 (1 reviews)

Novus Biologicals | Catalog # NBP2-61478

(1)

Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG₁ Clone # RMH01 expressed in HEK293

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Product Specifications

Immunogen

This His Tag Antibody (RMH01) was developed against a mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis Tag at the C-terminus. This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of NBP2-61482, with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.

Clonality

Monoclonal

Host

Human

Isotype

IgG₁

Scientific Data Images for His Tag Antibody (RMH01) - Chimeric

Western Blot: His Tag Antibody (RMH01) [NBP2-61478] -

Western Blot: His Tag Antibody (RMH01) [NBP2-61478] - Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using NBP2-61478 at 0.2 ug/mL, followed by a HRP conjugated anti-Human IgG secondary antibody.

Sandwich ELISA: His Tag Antibody (RMH01) - Chimeric - Azide and BSA Free [NBP2-61478] -

Sandwich ELISA: His Tag Antibody (RMH01) - Chimeric - Azide and BSA Free [NBP2-61478] - Sandwich ELISA performed on different amounts of whole lysate of 293T cells transfected with a DNA construct encoding His Tag Histone H3 (K36M) protein, using Clone RMH01 as the capture antibody. Another Histone H3 [monomethyl Lys 36] rabbit monoclonal antibody was used as the detection antibody, followed by an alkaline phosphatase conjugated Rabbit IgG as the Secondary antibody.

ELISA: His Tag Antibody (RMH01) - Chimeric - Azide and BSA Free [NBP2-61478] -

ELISA: His Tag Antibody (RMH01) - Chimeric - Azide and BSA Free [NBP2-61478] - A titer ELISA performed on purified His Tag-PCSK9 recombinant protein. A serial dilution of Clone RMH01 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody.

Applications for His Tag Antibody (RMH01) - Chimeric

Application

Recommended Usage

Sandwich ELISA Capture

0.1 - 2 ug/ml

Sandwich ELISA Detection

0.01 - 0.5 ug/ml

Western Blot

0.1 ug/ml - 1 ug/ml

Application Notes

See Simple Western Antibody Database for Simple Western validation: Tested in Human purified protein, separated by Size

Reviewed Applications

Read 1 review rated 5 using NBP2-61478 in the following applications:

Simple Western (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

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Product Specific Notices for His Tag Antibody (RMH01) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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His Tag Antibody (RMH01)

Name: Anonymous

Application: Simple Western

Sample Tested: Purified protein

Species: Human

Verified Customer | Posted 12/04/2018

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