HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72479

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C9

Format

Azide and BSA Free
View all HIST1H2BA Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 2-127 of human HIST1H2BA(NP_733759) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free

Western Blot: HIST1H2BA Antibody (OTI1C9)Azide and BSA Free [NBP2-72479]

Western Blot: HIST1H2BA Antibody (OTI1C9)Azide and BSA Free [NBP2-72479]

Western Blot: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIST1H2BA.
Immunohistochemistry: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479]

Immunohistochemistry: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479]

Immunohistochemistry: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479]

Immunohistochemistry: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479]

Immunohistochemistry: HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free [NBP2-72479] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIST1H2BA

HIST1H2BA is 127 amino acids long, weighs approximately 14 kDa, and the gene codes for a part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, papillary adenoma, adenoma, and immunodeficiency. HIST1H2BA has also been shown to have interactions with BRD7, DNTTIP2, HIST2H2AC, SET, and CCNA1 in pathways such as the telomere maintenance, nucleosome assembly, cell cycle, FOXM1 transcription factor, and systemic lupus erythematosus pathways.

Alternate Names

bA317E16.3, H2BFU, histone cluster 1, H2ba, histone H2B type 1-A, STBP, TSH2B

Gene Symbol

HIST1H2BA

Additional HIST1H2BA Products

Product Documents for HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HIST1H2BA Antibody (OTI1C9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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