HIST1H3A Antibody (CL4968) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-44008
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL4968
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of P68431, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for HIST1H3A Antibody (CL4968) - Azide and BSA Free
![HIST1H3A Antibody (CL4968) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]](https://resources.rndsystems.com/images/products/nbp3-44008_mouse-hist1h3a-mab-cl4968-azide-and-bsa-free-282202512405527.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]
Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells as well as in cells in lamina propria.![HIST1H3A Antibody (CL4968) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]](https://resources.rndsystems.com/images/products/nbp3-44008_mouse-hist1h3a-mab-cl4968-azide-and-bsa-free-282202512384288.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]
Staining of human fallopian tube shows strong nuclear positivity in glandular and stromal cells.![HIST1H3A Antibody (CL4968) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]](https://resources.rndsystems.com/images/products/nbp3-44008_mouse-hist1h3a-mab-cl4968-azide-and-bsa-free-28220251240558.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]
Staining of human cerebral cortex shows moderate to strong nuclear immunoreactivity in neuronal and glial cells.![HIST1H3A Antibody (CL4968) - Azide and BSA Free Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]](https://resources.rndsystems.com/images/products/nbp3-44008_mouse-hist1h3a-mab-cl4968-azide-and-bsa-free-28220251375647.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP3-44008]
Staining of human prostate shows moderate nuclear positivity in glandular and smooth muscle cells.Applications for HIST1H3A Antibody (CL4968) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: HIST1H3A
Alternate Names
H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Gene Symbol
H3C1
Additional HIST1H3A Products
Product Documents for HIST1H3A Antibody (CL4968) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HIST1H3A Antibody (CL4968) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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