Histamine H3R Antibody (7H8E6)

Novus Biologicals | Catalog # NBP3-16202

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7H8E6 expressed in HEK293
View all Histamine H3 R Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histamine H3R (Q9Y5N1). MERAPPDGPLNASGALAGEAAAAGGARGFSAAWTAVLAALMALLIVATVLGNALVMLAFVADSSLRTQNNFFLLNLAISDFLVGAFCIPLYVPYVLTGRW

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histamine H3R Antibody (7H8E6)

Histamine H3R Antibody (7H8E6)

Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] -

Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Western blot analysis of various lysates using Histamine H3R Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Histamine H3R Antibody (7H8E6)

Immunoprecipitation: Histamine H3R Antibody (7H8E6) [NBP3-16202] -

Immunoprecipitation: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Immunoprecipitation analysis of 300 ug extracts of Raji cells using 3 ug Histamine H3R antibody. Western blot was performed from the immunoprecipitate using Histamine H3R antibody at a dilution of 1:1000.
Histamine H3R Antibody (7H8E6)

Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] -

Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Western blot analysis of various lysates using Histamine H3R Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for Histamine H3R Antibody (7H8E6)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histamine H3 R

H3 Histamine Receptor regulates the release of the neurotransmitters norepinephrine and somatostatin. In histamine-containing neurons of the brain, the H3 receptor acts as a presynaptic autoreceptor, while in nonhistamine-containing neurons in both the central and peripheral nervous systems, it acts as a presynaptic heteroreceptor. Multiple isoforms of H3 receptors are produced by alternative splicing. Histamine H3 receptor has been reported primarily in brain. ESTs have been isolated from brain (normal and cancer), breast cancer, colon cancer, and retinoblastoma libraries.

Long Name

Histamine H3 Receptor

Alternate Names

GPCR97, HH3R, Histamine H3R, HRH3

Gene Symbol

HRH3

Additional Histamine H3 R Products

Product Documents for Histamine H3R Antibody (7H8E6)

Certificate of Analysis

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Product Specific Notices for Histamine H3R Antibody (7H8E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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