Histamine H4R Antibody - BSA Free

Novus Biologicals | Catalog # NLS3775

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Human

Predicted:

Bovine (94%), Equine (94%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histamine H4R Primary Antibodies »

Product Specifications

Immunogen

Synthetic 18 amino acid peptide from 1st cytoplasmic domain of human Histamine H4R.

Epitope

1st cytoplasmic domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Marmoset (100%), Gibbon (89%), Mouse (89%), Canine (89%), Rat (83%), Opossum (83%).

Localization

Integral membrane protein.

Specificity

Human Histamine H4R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Histamine H4R Antibody - BSA Free

Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Lung, Non Small-Cell Carcinoma
Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - IHC analysis of Lymph node using this antibody at 12 ug/ ml.
Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - Anti-HRH4 / Histamine H4 Receptor antibody IHC of human Ovary, Carcinoma and Inflammatory Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Ovary, carcinoma
Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Pancreas, carcinoma
Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775]

Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Colon, Carcinoma
Histamine H4R Antibody - BSA Free

Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775] -

Immunohistochemistry-Paraffin: Histamine H4R Antibody - BSA Free [NLS3775] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human Testis, Mast Cells tissue
Histamine H4R Antibody - BSA Free

Immunohistochemistry: Rabbit Histamine H4R pAb - BSA Free [NLS3775]

H4R expression was detected on a proportion of enterocytes, with enteroendocrine cell morphology, in the healthy human colon. Further, H4R was detected on mononuclear cells within the lamina propria of the ascending colon, as previously described.1:75 dilution in 4% PFA-fixed, frozen human colon samples. Primary antibody mix contained 0.2% Triton X-100. Image from a verified customer review.

Applications for Histamine H4R Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

20 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Histamine H4R

Histamine Receptor H4 binds histamine and several psychoactive compounds such as amitriptyline, chlorpromazine, cyproheptadine, and mianserin. The Histamine H4 receptor has been reported in human blood peripheral leukocytes, bone marrow, colon, liver, lung, smalll intestine, spleen, testis, thymus, tonsil, and trachea. Contrary to the mouse H4 receptor, the human gene was not detected in brain.

Long Name

Histamine H4 Receptor

Alternate Names

AXOR35, BG26, GPCR105, GPRv53, HH4R, Histamine H4 R, HRH4, Pfi-013, SP9144

Entrez Gene IDs

59340 (Human)

Gene Symbol

HRH4

UniProt

Q9H3N8 (Human)

Additional Histamine H4R Products

Product Documents for Histamine H4R Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Histamine H4R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Histamine H4R Antibody - BSA Free

Customer Reviews for Histamine H4R Antibody - BSA Free (1)

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  • Name: Anonymous
    Verified Customer | Posted 10/16/2025
    H4R expression was detected on a proportion of enterocytes, with enteroendocrine cell morphology, in the healthy human colon. Further, H4R was detected on mononuclear cells within the lamina propria of the ascending colon, as previously described
    1:75 dilution in 4% PFA-fixed, frozen human colon samples. Primary antibody mix contained 0.2% Triton X-100.
    Histamine H4R Antibody - BSA Free NLS3775

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Protocols

View specific protocols for Histamine H4R Antibody - BSA Free (NLS3775):

Immunohistochemistry Protocol for Histamine H4 Receptor Antibody (NLS3775):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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