Histamine N-Methyltransferase/HNMT Antibody (3G12) - Azide and BSA Free

Novus Biologicals | Catalog # H00003176-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3G12

Format

Azide and BSA Free
View all Histamine N-Methyltransferase/HNMT Primary Antibodies »

Product Specifications

Immunogen

HNMT (NP_008826, 184 a.a. ~ 292 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLLWDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA

Specificity

HNMT - histamine N-methyltransferase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images

Western Blot: Histamine N-Methyltransferase/HNMT Antibody (3G12) [H00003176-M03]

Western Blot: Histamine N-Methyltransferase/HNMT Antibody (3G12) [H00003176-M03]

Western Blot: Histamine N-Methyltransferase/HNMT Antibody (3G12) [H00003176-M03] - Analysis of HNMT expression in HeLa (Cat # L013V1).
ELISA: Histamine N-Methyltransferase/HNMT Antibody (3G12) [H00003176-M03]

ELISA: Histamine N-Methyltransferase/HNMT Antibody (3G12) [H00003176-M03]

ELISA: Histamine N-Methyltransferase/HNMT Antibody (3G12) [H00003176-M03] - Detection limit for recombinant GST tagged HNMT is approximately 0.1ng/ml as a capture antibody.

Applications

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histamine N-Methyltransferase/HNMT

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Long Name

Histamine N-Methyltransferase

Alternate Names

Histamine NMethyltransferase, HMT, HNMT, HNMT-S1, HNMT-S2

Entrez Gene IDs

3176 (Human)

Gene Symbol

HNMT

OMIM

600807 (Human)

UniProt

NP_008826 (Human)

Additional Histamine N-Methyltransferase/HNMT Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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