Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16793

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone Deacetylase 4/HDAC4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone Deacetylase 4/HDAC4. The exact sequence is proprietary.

Reactivity Notes

Chicken (85%).

Localization

Nucleus; Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

119 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody, Internal (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HDAC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Human colon carcinoma, using HDAC4 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Knockdown Validated: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793]

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Immunoprecipitation: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] -

HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of HDAC4 protein by 4 ug HDAC4 antibody [N3C1], Internal (NBP2-16793)
5 % SDS-PAGE
The immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal (NBP2-16793) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (NBP2-16793) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
IHC-FR-Assay dependent

Reviewed Applications

Read 1 review rated 5 using NBP2-16793 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 4/HDAC4

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq]

Long Name

Histone Deacetylase 4

Alternate Names

HDAC4

Gene Symbol

HDAC4

UniProt

P56524 (Human)

Additional Histone Deacetylase 4/HDAC4 Products

Product Documents for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis
Epigenetics

Citations for Histone Deacetylase 4/HDAC4 Antibody - BSA Free

Customer Reviews for Histone Deacetylase 4/HDAC4 Antibody - BSA Free (1)

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  • Name: Liyong Zhang
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 08/15/2014
    A review for HDAC4 antibody (NBP2-16793)
    Histone Deacetylase 4/HDAC4 Antibody - BSA Free NBP2-16793

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