Histone Deacetylase 8/HDAC8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-14085

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone Deacetylase 8/HDAC8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: VLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone Deacetylase 8/HDAC8 Antibody - BSA Free

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085] - Analysis in human thyroid gland and skeletal muscle tissues. Corresponding HDAC8 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085] - Staining of human cell line PC-3 shows localization to nucleus & plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085] - Staining of human rectum shows moderate to strong nuclear positivity in glandular cells, as well as in lymphoid cells.
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085] - Staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085]

Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody [NBP2-14085] - Staining of human prostate shows moderate nuclear positivity in glandular cells.

Applications for Histone Deacetylase 8/HDAC8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 8/HDAC8

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.

Alternate Names

HDAC8, HDACL1, RPD3

Gene Symbol

HDAC8

Additional Histone Deacetylase 8/HDAC8 Products

Product Documents for Histone Deacetylase 8/HDAC8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 8/HDAC8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Histone Deacetylase 8/HDAC8 Antibody - BSA Free

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Protocols

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