Histone H1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-748

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Mammal

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

Azide and BSA Free
View all Histone H1 Primary Antibodies »

Product Specifications

Immunogen

Calf thymus intact Histone H1 (MW 23 kDa) complexed to rRNA.

Reactivity Notes

Cross-reacts with all Mammals.

Localization

Nuclear

Marker

Nuclear Marker

Specificity

This specifically binds to the Histone H1 protein found in nucleosomes of all eukaryotic cells. Differences between H1 subfractions of one species are greater than the differences across species. No cross reactivity was observed with other Histone core proteins H2A, H2B, H3 or H4.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Histone H1 Antibody - Azide and BSA Free

Western Blot: Histone H1 Antibody [NB100-748]

Western Blot: Histone H1 Antibody [NB100-748]

Western Blot: Histone H1 Antibody [NB100-748] - The blot shows the antibody reactiving against porcine Histone H1. (Lane 1: negative control). Zhu G et al. j. Biol. Chem. 2005, 280:23057-23065

Applications for Histone H1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:20

Western Blot

1:300
Application Notes
Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone H1

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Alternate Names

H1 histone family, member 1, H1.1, H1A, H1F1HIST1, histone 1, H1a, histone cluster 1, H1a, histone H1.1, MGC126642, MGC138345

Gene Symbol

H1-1

Additional Histone H1 Products

Product Documents for Histone H1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Histone H1 Antibody - Azide and BSA Free

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Protocols

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FAQs

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