Histone H2A.Z Antibody (2X2I10)

Name: Histone H2A.Z Antibody (2X2I10)
Brand: Novus Biologicals
SKU: NBP3-16447
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16447

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2X2I10 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2A.Z (P0C0S5). MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2A.Z Antibody (2X2I10)

Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447]

Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Western blot analysis of extracts of various cell lines, using Histone H2A.Z Rabbit mAb (NBP3-16447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] -

Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2A.Z Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] -

Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Western blot analysis of lysates from wild type(WT) and Histone H2A.Z knockout (KO) 293T(KO) cells, using [KO Validated] Histone H2A.Z Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [Histone H2A.Z] -

Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [Histone H2A.Z] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2A.Z Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Applications for Histone H2A.Z Antibody (2X2I10)

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:50-1:200

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2A.Z

Alternate Names

H2A histone family, member Z, H2A.Z, H2A/z, H2AZ histone, H2AZMGC117173, histone H2A.Z

Gene Symbol

H2AZ1

Additional Histone H2A.Z Products

Product Documents for Histone H2A.Z Antibody (2X2I10)

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Certificate of Analysis

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Product Specific Notices for Histone H2A.Z Antibody (2X2I10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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