Histone H2A.Z Antibody (2X2I10)
Novus Biologicals | Catalog # NBP3-16447
Recombinant Monoclonal Antibody
![Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447]](https://resources.rndsystems.com/images/products/Histone-H2A-Z-Antibody-2X2I10-Western-Blot-NBP3-16447-img0001.jpg "Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Loading...
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2X2I10 expressed in HEK293
Loading...
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2A.Z (P0C0S5). MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2A.Z Antibody (2X2I10)
Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447]
Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Western blot analysis of extracts of various cell lines, using Histone H2A.Z Rabbit mAb (NBP3-16447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] -
Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2A.Z Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] -
Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Western blot analysis of lysates from wild type(WT) and Histone H2A.Z knockout (KO) 293T(KO) cells, using [KO Validated] Histone H2A.Z Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [Histone H2A.Z] -
Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [Histone H2A.Z] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2A.Z Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Applications for Histone H2A.Z Antibody (2X2I10)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:50-1:200
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2A.Z
Alternate Names
H2A histone family, member Z, H2A.Z, H2A/z, H2AZ histone, H2AZMGC117173, histone H2A.Z
Gene Symbol
H2AZ1
Additional Histone H2A.Z Products
Product Documents for Histone H2A.Z Antibody (2X2I10)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histone H2A.Z Antibody (2X2I10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Histone H2A.Z Antibody (2X2I10)
There are currently no reviews for this product. Be the first to review Histone H2A.Z Antibody (2X2I10) and earn rewards!
Have you used Histone H2A.Z Antibody (2X2I10)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...