Histone H3.3 Antibody (RM190) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-26046
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Vertebrate
Applications
Multiplex Immunofluorescence, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM190 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
A peptide corresponding to Human Histone H3.3
Specificity
This antibody reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H3.3 Antibody (RM190) - Azide and BSA Free
Western Blot: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] -
Western Blot: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] - Western Blot of HeLa whole cell lysate, recombinant Histone H3.3, and Histone H3.1 proteins, using RM190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.Multiplex Immunoassay: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] -
Multiplex Immunoassay: Histone H3.3 Antibody (RM190) - Azide and BSA Free [NBP3-26046] - RM190 reacts specifically to Histone H3.3. No cross reactivity with Histone H3.1.Applications for Histone H3.3 Antibody (RM190) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.2 ug/mL - 1 ug/mL
Western Blot
0.5 ug/ml - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H3.3
Alternate Names
H3.3A, H3F3, H3F3A, Histone H3.3, Histone H3.3A, Histone H3.3B, MGC87782, MGC87783
Gene Symbol
H3-3A
Additional Histone H3.3 Products
Product Documents for Histone H3.3 Antibody (RM190) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histone H3.3 Antibody (RM190) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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