Histone H3.3B Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-24899

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone H3.3B Primary Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein Histone H3.3B using the following amino acid sequence: KPHRYRPGTVALREIRRYQKSTELLIR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H3.3B Antibody - BSA Free

Histone H3.3B Antibody Immunocytochemistry/Immunofluorescence: Histone H3.3B Antibody [NBP3-24899]

Immunocytochemistry/Immunofluorescence: Histone H3.3B Antibody [NBP3-24899]

Staining of human cell line PC-3 shows localization to nucleoplasm.

Applications for Histone H3.3B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H3.3B

Histone H3.3B is 136 amino acids long, weighs approximately 15 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, malaria, leukemia, retinoblastoma, and immunodeficiency. Histone H3.3B has also been shown to have interactions with PELP1, HIST1H4A, HIST1H4B, HIST1H4C, and HIST1H4D in pathways such as the histone modification, development notch signaling, signal transduction for Activin A, amyloids, hemostasis, meiosis, meiotic recombination, and systemic lupus erythematosus pathways.

Alternate Names

H3 histone, family 3A, H3 histone, family 3B (H3.3B), H3.3BH3.3A, H3F3, H3F3A, histone H3.3

Gene Symbol

H3-3B

Additional Histone H3.3B Products

Product Documents for Histone H3.3B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H3.3B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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