The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
HIV-1 Gag p24 Antibody (7F4) - BSA Free
Novus Biologicals | Catalog # NBP2-41339
Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7F4
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a recombinant full-length HIV-1 Gag p24 protein.
Specificity
By Western blot, anti-HIV-1 Gag p24 antibody detects a ~24 kDa, a ~41 kDa, and a ~55 kDa protein, corresponding to HIV-1 Gag p24 and to its precursors p41 and p55, respectively, in HIV-1 samples.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
24, 41, 55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HIV-1 Gag p24 Antibody (7F4) - BSA Free
Western Blot: HIV-1 Gag p24 Antibody (7F4)BSA Free [NBP2-41339]
Western Blot: HIV-1 Gag p24 Antibody (7F4) - BSA Free [NBP2-41339] - analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 Gag p24 antibody PM-6585 at (A) 0.5 ug/mL and (B) 0.2 ug/mL, respectively.ELISA: HIV-1 Gag p24 Antibody (7F4) - BSA Free [NBP2-41339]
ELISA: HIV-1 Gag p24 Antibody (7F4) - BSA Free [NBP2-41339] - Titration ELISA of anti-HIV-1 Gag p24 antibody PM-6585 with 100 ng of recombinant HIV-1 Gag p24 protein.ELISA: HIV-1 Gag p24 Antibody (7F4) - BSA Free [NBP2-41339]
ELISA: HIV-1 Gag p24 Antibody (7F4) - BSA Free [NBP2-41339] - Analysis of recombinant p24 proteins using capture antibody [7F4] and biotin labeled detection antibody [8G9]. (Assay sensitivity = 2.5 pg of recombinant p24 protein).Applications for HIV-1 Gag p24 Antibody (7F4) - BSA Free
Application
Recommended Usage
ELISA
0.2- 0.5 ug/mL
Western Blot
0.2-0.5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HIV-1 Gag p24
Long Name
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Alternate Names
CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)
Gene Symbol
gag
Additional HIV-1 Gag p24 Products
Product Documents for HIV-1 Gag p24 Antibody (7F4) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HIV-1 Gag p24 Antibody (7F4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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