The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
HIV-1 Gag p24 Antibody (9B6C2B7) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-06452
Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 9B6C2B7
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant HIV-1 Gag p24 (Accession#: ACI05538.1; Pro 133-Leu 363).
Reactivity Notes
No cross-reactivity in ELISA with:
E.coli cell lysate
E.coli cell lysate
Specificity
No cross-reactivity in ELISA with: E.coli cell lysate
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Applications for HIV-1 Gag p24 Antibody (9B6C2B7) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:1000-1:2000
Western Blot
1:500-1:1000
Application Notes
Western Blot: Using a DAB detection system, the detection limit for HIV-1 Gag p24 is approximately 20 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HIV-1 Gag p24
Long Name
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Alternate Names
CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)
Gene Symbol
gag
Additional HIV-1 Gag p24 Products
Product Documents for HIV-1 Gag p24 Antibody (9B6C2B7) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HIV-1 Gag p24 Antibody (9B6C2B7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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