< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
QC221 , Quantikine Immunoassay Control Set 896 Human HIV-1 Gag p24 -
The Quantikine HIV-1 Gag p24 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure HIV-1 Gag p24 in cell culture supernates, serum, and plasma.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
The recovery of HIV-1 Gag p24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
Average % Recovery
Cell Culture Media (n=4)
EDTA Plasma (n=4)
EDTA Plasma (ICD Treated) (n=4)
Heparin Plasma (n=4)
Heparin Plasma (ICD Treated) (n=4)
To assess the linearity of the assay, samples spiked with high concentrations of HIV-1 Gag p24 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HIV-1 Gag p24
The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
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