HIV-1 Gag p24 Quantikine ELISA Kit

  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    3.25 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    HIV-1 Gag p24.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC221, Quantikine Immunoassay Control Set 896 Human HIV-1 Gag p24 - Please Inquire
Product Summary
The Quantikine HIV-1 Gag p24 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure HIV-1 Gag p24 in cell culture supernates, serum, and plasma.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean45.713528043132285
Standard Deviation1.414.868.11.934.6111.2
CV%3.13.62.94.53.53.9

Recovery

The recovery of HIV-1 Gag p24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 93-105
EDTA Plasma (n=4) 74 64-82
EDTA Plasma (ICD Treated) (n=4) 92 85-100
Heparin Plasma (n=4) 72 63-78
Heparin Plasma (ICD Treated) (n=4) 88 69-94
Serum (n=4) 79 73-85
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of HIV-1 Gag p24 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
HIV-1 Gag p24 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HIV-1 Gag p24
The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
    • Long Name
      Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
    • Entrez Gene IDs
      155030 (Viral);
    • Alternate Names
      CA; Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag);
    Related Research Areas
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 896 Human HIV-1 Gag p24

    Ctrl QC221
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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