HIV-1 Gag p24 Quantikine ELISA Kit Summary
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of HIV-1 Gag p24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||100||93-105|
|EDTA Plasma (n=4)||74||64-82|
|EDTA Plasma (ICD Treated) (n=4)||92||85-100|
|Heparin Plasma (n=4)||72||63-78|
|Heparin Plasma (ICD Treated) (n=4)||88||69-94|
Preparation and Storage
Background: HIV-1 Gag p24
The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
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