HLA A Antibody (2D6) - Azide and BSA Free

Novus Biologicals | Catalog # H00003105-M01-100ug

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D6

Format

Azide and BSA Free
View all HLA A Primary Antibodies »

Product Specifications

Immunogen

HLA-A (AAH03069, 24 a.a. ~ 365 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQPTIPIVGIIAGLVLLGAVITGAVVAAVMWRRKSSDRKGGSYTQAASSDSAQGSDVSLTACKV

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for HLA A Antibody (2D6) - Azide and BSA Free

Western Blot: HLA A Antibody (2D6) [H00003105-M01-100ug]

Western Blot: HLA A Antibody (2D6) [H00003105-M01-100ug]

Western Blot: HLA A Antibody (2D6) [H00003105-M01-100ug] - Detection against Immunogen (63.36 KDa).
Western Blot: HLA A Antibody (2D6) [H00003105-M01-100ug]

Western Blot: HLA A Antibody (2D6) [H00003105-M01-100ug]

Western Blot: HLA A Antibody (2D6) [H00003105-M01-100ug] - Analysis of HLA-A expression in transfected 293T cell line by HLA-A monoclonal antibody (M01), clone 2D6. Lane 1: HLA-A transfected lysate (Predicted MW: 40.8 KDa). Lane 2: Non-transfected lysate
Western Blot: HLA A Antibody (2D6) [H00003105-M01-100ug]

Western Blot: HLA A Antibody (2D6) [H00003105-M01-100ug]

Western Blot: HLA A Antibody (2D6) [H00003105-M01-100ug] - Analysis of HLA-A expression in A-431.
ELISA: HLA A Antibody (2D6) [H00003105-M01-100ug]

ELISA: HLA A Antibody (2D6) [H00003105-M01-100ug]

ELISA: HLA A Antibody (2D6) [H00003105-M01-100ug] - Detection limit for recombinant GST tagged HLA-A is 3 ng/ml as a capture antibody.

Applications for HLA A Antibody (2D6) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLA A

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq]

Alternate Names

A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80

Gene Symbol

HLA-A

OMIM

106300 (Human)

UniProt

AAH03069 (Human)

Additional HLA A Products

Product Documents for HLA A Antibody (2D6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HLA A Antibody (2D6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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