Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, ELISA, Flow Cytometry, Immunoprecipitation, Functional, Immunohistochemistry-Paraffin (Negative), CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunoprecipitation, Magnetic Cell Separation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # W6/32

Format

Azide and BSA Free
View all HLA ABC Primary Antibodies »

Product Specifications

Immunogen

Purified human tonsil lymphocyte membranes

Reactivity Notes

Predicted cross-reactivities: Cynomolgus monkey, Chimpanzee, Baboon, Bovine, Macaque, Gorilla, Shrew, Rhesus Monkey

Specificity

NB100-64775 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for HLA ABC Antibody (W6/32) - Azide and BSA Free

Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution. Image from verified customer review.
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488.
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647.
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC.
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin.
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Human lymphoblast. Image provided via verified customer review.
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of peripheral blood lymphocytes with anti-HLA-ABC: Low Endotoxin.
Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) - Azide and BSA Free [NB100-64775]

Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the PE/Cy5 conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cy5 (NBP1-43073) (blue histogram) or Anti-Human HLA-ABC PE-Cy5 (purple histogram). Cells in the lymphocyte gate were

Applications for HLA ABC Antibody (W6/32) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
Antibody does not work in Immunohistochemistry-Paraffin.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-64775 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HLA ABC

HLA CLass I is involved in the presentation of foreign antigens to the immune system. HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

Alternate Names

A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80

Gene Symbol

HLA-A

Additional HLA ABC Products

Product Documents for HLA ABC Antibody (W6/32) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HLA ABC Antibody (W6/32) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HLA ABC Antibody (W6/32) - Azide and BSA Free

Customer Reviews for HLA ABC Antibody (W6/32) - Azide and BSA Free (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • HLA ABC Antibody (W6/32)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Colon cancer
    Species: Mouse
    Verified Customer | Posted 01/31/2022
    Mouse colon cancer tissue
    Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution
    HLA ABC Antibody (W6/32) - Azide and BSA Free NB100-64775
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Cell Line, lymphoblast
    Species: Human
    Verified Customer | Posted 02/28/2014
    HLA B27 expression study
    HLA ABC Antibody (W6/32) - Azide and BSA Free NB100-64775

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HLA ABC Antibody (W6/32) - Azide and BSA Free

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  • Q: We have purchased the antibody NB100-64775 HLA ABC Antibody (W6/32) 0.25mg. To date we have completed over 40 reactions and haven't found a single positive output. Can you please send me the flow cytometry images generated by this antibody??

    A:

    I'm sorry to hear about the dificulty you have been having. Please refer to the images section of the datasheet. There are also many publications on the product page for your review. I did find PubMeb 21123379 links to a free PMC article that talks about this clone being used in Flow Cytometry. We also offer many conjugated forms of this antibody that you may be interested in. You can always contact technical@novusbio.com with any additional questions.

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