HLCS Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32174

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HLCS Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Holocarboxylase Synthetase using the numbering given in entry NP_001229714.1 (GeneID 3141).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLCS Antibody - BSA Free

Western Blot: HLCS Antibody [NBP2-32174]

Western Blot: HLCS Antibody [NBP2-32174]

Western Blot: HLCS Antibody [NBP2-32174] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-HLCS antibody NBP2-32174 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: HLCS Antibody [NBP2-32174]

Immunoprecipitation: HLCS Antibody [NBP2-32174]

Immunoprecipitation: HLCS Antibody [NBP2-32174] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HLCS antibody NBP2-32174 used for IP at 6 ug per reaction. HLCS was also immunoprecipitated by rabbit anti-HLCS antibody BL15481. For blotting immunoprecipitated HLCS, NBP2-32174 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for HLCS Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HLCS

Holocarboxylase synthetase (EC 6.3.4.10) covalently links biotin to propionyl-CoA carboxylase (PCCA; MIM 232000), pyruvate carboxylase (PC; MIM 608786), alpha-methylcrotonyl-CoA carboxylase (MCCC1; MIM 609010), and acetyl-CoA carboxylase (ACACA; MIM 200350).[supplied by OMIM]

Alternate Names

Biotin apo-protein ligase, biotin--protein ligase, EC 6.3.4.-, HCSholocarboxylase synthetase, EC 6.3.410holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)) ligase), holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase(ATP-hydrolysing)) ligase), holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)] ligase)

Gene Symbol

HLCS

UniProt

NP_001229714.1 (Human)

Additional HLCS Products

Product Documents for HLCS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HLCS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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