HLTF Antibody

Novus Biologicals | Catalog # NB100-1041

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Primate

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HLTF Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KQAKINEIRTLIDL corresponding to C-Terminus according to NP_003062.2, NP_620636.1.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 23415230). Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Specificity

NP_003062.2 and NP_620636.2 are varients that represent the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HLTF Antibody

Western Blot: HLTF Antibody [NB100-1041]

Western Blot: HLTF Antibody [NB100-1041]

Western Blot: HLTF Antibody [NB100-1041] - staining (0.5ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
Flow Cytometry: HLTF Antibody [NB100-1041]

Flow Cytometry: HLTF Antibody [NB100-1041]

Flow Cytometry: HLTF Antibody [NB100-1041] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for HLTF Antibody

Application
Recommended Usage

Flow Cytometry

10ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3-1ug/ml
Application Notes
Western blot: Approx. 125kDa band observed in nuclear lysates of cell line HeLa and in preliminary testing of A431 nuclear cell lysate (calculated MW of 114kDa according to NP_003062.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HLTF

The HLTF (Helicase-like Transcription Factor; SMARCA 3) gene is a member of the family of genes that remove proteins that coil around DNA. HLTF proteins aid the stabilization and regulation of the production of proteins in the cell. Inactivation of the HTLF gene may contribute to the transformation of normal colon cells into cancer cells.

Alternate Names

DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC 3.6.1, EC 3.6.4.-, EC 6.3.2.-, helicase-like transcription factor, HIP116ARING finger protein 80, HIP116matrix associated, actin dependent regulator of chromatin, RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3, subfamily a, member 3, Sucrose nonfermenting protein 2-like 3, ZBU1

Entrez Gene IDs

6596 (Human); 20585 (Mouse)

Gene Symbol

HLTF

UniProt

Q14527 (Human)

Additional HLTF Products

Product Documents for HLTF Antibody

Certificate of Analysis

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Product Specific Notices for HLTF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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