HMBS Antibody (3E8) - Azide and BSA Free

Novus Biologicals | Catalog # H00003145-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E8

Format

Azide and BSA Free
View all HMBS Primary Antibodies »

Product Specifications

Immunogen

HMBS (AAH00520.1, 1 a.a. ~ 361 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKIPDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH

Specificity

HMBS (3E8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HMBS Antibody (3E8) - Azide and BSA Free

Western Blot: HMBS Antibody (3E8) [H00003145-M01]

Western Blot: HMBS Antibody (3E8) [H00003145-M01]

Western Blot: HMBS Antibody (3E8) [H00003145-M01] - Analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.Lane 1: HMBS transfected lysate(39.71 KDa).Lane 2: Non-transfected lysate.
Western Blot: HMBS Antibody (3E8) [H00003145-M01]

Western Blot: HMBS Antibody (3E8) [H00003145-M01]

Western Blot: HMBS Antibody (3E8) [H00003145-M01] - analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.
Sandwich ELISA: HMBS Antibody (3E8) [H00003145-M01] - Detection limit for recombinant GST tagged HMBS is approximately 1ng/ml as a capture antibody.

Applications for HMBS Antibody (3E8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HMBS

This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

hydroxymethylbilane synthasePre-uroporphyrinogen synthase, PBGDPBG-D, PORC, porphobilinogen deaminase, porphyria, acute; Chester type, UPSEC 2.5.1.61, uroporphyrinogen I synthase, uroporphyrinogen I synthetase

Entrez Gene IDs

3145 (Human)

Gene Symbol

HMBS

OMIM

609806 (Human)

UniProt

AAH00520 (Human)

Additional HMBS Products

Product Documents for HMBS Antibody (3E8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HMBS Antibody (3E8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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